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− | ==OVERVIEW== | + | == OVERVIEW == |
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| The SEM Operation checklist in Section 2 contains step by step instructions to operate the SEM. Note that these steps reference Procedures described in [[#PROCEDURES|PROCEDURES]]. These are listed in alphabetical order. For troubleshooting, see [[#TROUBLESHOOTING|TROUBLESHOOTING]]. | | The SEM Operation checklist in Section 2 contains step by step instructions to operate the SEM. Note that these steps reference Procedures described in [[#PROCEDURES|PROCEDURES]]. These are listed in alphabetical order. For troubleshooting, see [[#TROUBLESHOOTING|TROUBLESHOOTING]]. |
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− | ==SEM OPERATION== | + | == SEM OPERATION == |
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− | ===STARTUP=== | + | === STARTUP === |
| * Verify power switch in Standby, vacuum pumps running. | | * Verify power switch in Standby, vacuum pumps running. |
| ** If not, go to [[#POWER / VACUUM TROUBLESHOOTING|POWER / VACUUM TROUBLESHOOTING]]. | | ** If not, go to [[#POWER / VACUUM TROUBLESHOOTING|POWER / VACUUM TROUBLESHOOTING]]. |
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| * Set Tilt to 0 | | * Set Tilt to 0 |
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− | ===BEAM ON=== | + | === BEAM ON === |
| * Press Stoplight icon. | | * Press Stoplight icon. |
| * Wait for image. | | * Wait for image. |
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| * If an aperture other than #2 is required, follow [[#CHANGE APERTURE|CHANGE APERTURE]] procedure. NOTE: #2 aperture is usually the best aperture, so changing apertures isn’t necessary. | | * If an aperture other than #2 is required, follow [[#CHANGE APERTURE|CHANGE APERTURE]] procedure. NOTE: #2 aperture is usually the best aperture, so changing apertures isn’t necessary. |
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− | ===OPERATION=== | + | === OPERATION === |
| * Move sample as required to center item of interest. | | * Move sample as required to center item of interest. |
| * Zoom (Magnifying glass icon, left mouse button) as required. | | * Zoom (Magnifying glass icon, left mouse button) as required. |
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| ** Export images as needed. (see [[#EXPORT IMAGE|EXPORT IMAGE]] procedure) | | ** Export images as needed. (see [[#EXPORT IMAGE|EXPORT IMAGE]] procedure) |
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− | ===SHUTDOWN=== | + | === SHUTDOWN === |
| * Change to aperture #2 and align aperture if you previously changed to a different aperture. Follow [[#CHANGE APERTURE|CHANGE APERTURE]] procedure. | | * Change to aperture #2 and align aperture if you previously changed to a different aperture. Follow [[#CHANGE APERTURE|CHANGE APERTURE]] procedure. |
| * Set X to 50.00 | | * Set X to 50.00 |
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| * At DOS prompt, click Standby power button. | | * At DOS prompt, click Standby power button. |
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− | ==PROCEDURES== | + | == PROCEDURES == |
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− | ===ALIGN APERTURE=== | + | === ALIGN APERTURE === |
| * Zoom in to 1000x. | | * Zoom in to 1000x. |
| * Center on noticeable object or feature. | | * Center on noticeable object or feature. |
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| * Click Off button in focus wobble box. | | * Click Off button in focus wobble box. |
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− | ===CHANGE APERTURE=== | + | === CHANGE APERTURE === |
| * Beam -> Apertures. | | * Beam -> Apertures. |
| * Select aperture number. | | * Select aperture number. |
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| * Follow [[#ALIGN APERTURE|ALIGN APERTURE]] procedure. | | * Follow [[#ALIGN APERTURE|ALIGN APERTURE]] procedure. |
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− | ===EXPORT IMAGE=== | + | === EXPORT IMAGE === |
| * File -> Export TIFF | | * File -> Export TIFF |
| * Click Freeze if image not already frozen. | | * Click Freeze if image not already frozen. |
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| * Image -> Unfreeze | | * Image -> Unfreeze |
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− | ===FILAMENT CURRENT=== | + | === FILAMENT CURRENT === |
| * Scan rate to fastest. (Click Fast Forward icon 3 times) | | * Scan rate to fastest. (Click Fast Forward icon 3 times) |
| * Click light bulb icon. | | * Click light bulb icon. |
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| ** If well defined 2nd peak is visible, adjust for maximum brightness such that ANY decrease in current decreases image intensity. NOTE: Any operation above this point even more drastically shortens filament life and must be avoided. | | ** If well defined 2nd peak is visible, adjust for maximum brightness such that ANY decrease in current decreases image intensity. NOTE: Any operation above this point even more drastically shortens filament life and must be avoided. |
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− | ===FOCUS=== | + | === FOCUS === |
| * Consider anti-hysteresis (F2) after major parameter changes. (zoom changes | | * Consider anti-hysteresis (F2) after major parameter changes. (zoom changes |
| * Consider reduced raster. (Monitor icon with square, size and drag as appropriate) | | * Consider reduced raster. (Monitor icon with square, size and drag as appropriate) |
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| * NOTE: Auto-focus can be done via F key but it can sometimes make focus worse. | | * NOTE: Auto-focus can be done via F key but it can sometimes make focus worse. |
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− | ===NOISE REDUCTION=== | + | === NOISE REDUCTION === |
| * Scan rate to slowest. (Click Rewind icon 3 times) | | * Scan rate to slowest. (Click Rewind icon 3 times) |
| * Image -> Noise Reduction.. | | * Image -> Noise Reduction.. |
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| * Image -> Unfreeze. | | * Image -> Unfreeze. |
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− | ===PUSH IMAGES=== | + | === PUSH IMAGES === |
| * WARNING: Only do this when LEO UIF software is shut down! | | * WARNING: Only do this when LEO UIF software is shut down! |
| * Double click “Push [Your Name]” from list of icons in User Operations window. | | * Double click “Push [Your Name]” from list of icons in User Operations window. |
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| ** Repeat this procedure WITHOUT LAUNCHING LEO UIF. | | ** Repeat this procedure WITHOUT LAUNCHING LEO UIF. |
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− | ===PUMP CHAMBER=== | + | === PUMP CHAMBER === |
| * Press V (if vacuum window not open). | | * Press V (if vacuum window not open). |
| * Click Pump. | | * Click Pump. |
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| * If vacuum does not reach this level in 20 minutes, report to mailing list. | | * If vacuum does not reach this level in 20 minutes, report to mailing list. |
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− | ===VENT CHAMBER=== | + | === VENT CHAMBER === |
| * Press V (if vacuum window not open). | | * Press V (if vacuum window not open). |
| * Click Vent. | | * Click Vent. |
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− | ==TROUBLESHOOTING== | + | == TROUBLESHOOTING == |
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− | ===MOUSE TROUBLESHOOTING=== | + | === MOUSE TROUBLESHOOTING === |
| * In case of erratic mouse movement: | | * In case of erratic mouse movement: |
| ** Lift mouse, shake it, blow into track ball | | ** Lift mouse, shake it, blow into track ball |
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| ** Click anywhere else within the image. | | ** Click anywhere else within the image. |
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− | ===POWER / VACUUM TROUBLESHOOTING=== | + | === POWER / VACUUM TROUBLESHOOTING === |
| * If Off light is illuminated: | | * If Off light is illuminated: |
| ** SEM likely lost power. Report loss of power to mailing list. | | ** SEM likely lost power. Report loss of power to mailing list. |