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Leica S430 Scanning Electron Microscope Operations Checklist (view source)
Revision as of 00:15, 18 March 2014
, 00:15, 18 March 2014Robot: Cosmetic changes
==OVERVIEW==
== OVERVIEW ==
The SEM Operation checklist in Section 2 contains step by step instructions to operate the SEM. Note that these steps reference Procedures described in [[#PROCEDURES|PROCEDURES]]. These are listed in alphabetical order. For troubleshooting, see [[#TROUBLESHOOTING|TROUBLESHOOTING]].
The SEM Operation checklist in Section 2 contains step by step instructions to operate the SEM. Note that these steps reference Procedures described in [[#PROCEDURES|PROCEDURES]]. These are listed in alphabetical order. For troubleshooting, see [[#TROUBLESHOOTING|TROUBLESHOOTING]].
==SEM OPERATION==
== SEM OPERATION ==
===STARTUP===
=== STARTUP ===
* Verify power switch in Standby, vacuum pumps running.
* Verify power switch in Standby, vacuum pumps running.
** If not, go to [[#POWER / VACUUM TROUBLESHOOTING|POWER / VACUUM TROUBLESHOOTING]].
** If not, go to [[#POWER / VACUUM TROUBLESHOOTING|POWER / VACUUM TROUBLESHOOTING]].
* Set Tilt to 0
* Set Tilt to 0
===BEAM ON===
=== BEAM ON ===
* Press Stoplight icon.
* Press Stoplight icon.
* Wait for image.
* Wait for image.
* If an aperture other than #2 is required, follow [[#CHANGE APERTURE|CHANGE APERTURE]] procedure. NOTE: #2 aperture is usually the best aperture, so changing apertures isn’t necessary.
* If an aperture other than #2 is required, follow [[#CHANGE APERTURE|CHANGE APERTURE]] procedure. NOTE: #2 aperture is usually the best aperture, so changing apertures isn’t necessary.
===OPERATION===
=== OPERATION ===
* Move sample as required to center item of interest.
* Move sample as required to center item of interest.
* Zoom (Magnifying glass icon, left mouse button) as required.
* Zoom (Magnifying glass icon, left mouse button) as required.
** Export images as needed. (see [[#EXPORT IMAGE|EXPORT IMAGE]] procedure)
** Export images as needed. (see [[#EXPORT IMAGE|EXPORT IMAGE]] procedure)
===SHUTDOWN===
=== SHUTDOWN ===
* Change to aperture #2 and align aperture if you previously changed to a different aperture. Follow [[#CHANGE APERTURE|CHANGE APERTURE]] procedure.
* Change to aperture #2 and align aperture if you previously changed to a different aperture. Follow [[#CHANGE APERTURE|CHANGE APERTURE]] procedure.
* Set X to 50.00
* Set X to 50.00
* At DOS prompt, click Standby power button.
* At DOS prompt, click Standby power button.
==PROCEDURES==
== PROCEDURES ==
===ALIGN APERTURE===
=== ALIGN APERTURE ===
* Zoom in to 1000x.
* Zoom in to 1000x.
* Center on noticeable object or feature.
* Center on noticeable object or feature.
* Click Off button in focus wobble box.
* Click Off button in focus wobble box.
===CHANGE APERTURE===
=== CHANGE APERTURE ===
* Beam -> Apertures.
* Beam -> Apertures.
* Select aperture number.
* Select aperture number.
* Follow [[#ALIGN APERTURE|ALIGN APERTURE]] procedure.
* Follow [[#ALIGN APERTURE|ALIGN APERTURE]] procedure.
===EXPORT IMAGE===
=== EXPORT IMAGE ===
* File -> Export TIFF
* File -> Export TIFF
* Click Freeze if image not already frozen.
* Click Freeze if image not already frozen.
* Image -> Unfreeze
* Image -> Unfreeze
===FILAMENT CURRENT===
=== FILAMENT CURRENT ===
* Scan rate to fastest. (Click Fast Forward icon 3 times)
* Scan rate to fastest. (Click Fast Forward icon 3 times)
* Click light bulb icon.
* Click light bulb icon.
** If well defined 2nd peak is visible, adjust for maximum brightness such that ANY decrease in current decreases image intensity. NOTE: Any operation above this point even more drastically shortens filament life and must be avoided.
** If well defined 2nd peak is visible, adjust for maximum brightness such that ANY decrease in current decreases image intensity. NOTE: Any operation above this point even more drastically shortens filament life and must be avoided.
===FOCUS===
=== FOCUS ===
* Consider anti-hysteresis (F2) after major parameter changes. (zoom changes
* Consider anti-hysteresis (F2) after major parameter changes. (zoom changes
* Consider reduced raster. (Monitor icon with square, size and drag as appropriate)
* Consider reduced raster. (Monitor icon with square, size and drag as appropriate)
* NOTE: Auto-focus can be done via F key but it can sometimes make focus worse.
* NOTE: Auto-focus can be done via F key but it can sometimes make focus worse.
===NOISE REDUCTION===
=== NOISE REDUCTION ===
* Scan rate to slowest. (Click Rewind icon 3 times)
* Scan rate to slowest. (Click Rewind icon 3 times)
* Image -> Noise Reduction..
* Image -> Noise Reduction..
* Image -> Unfreeze.
* Image -> Unfreeze.
===PUSH IMAGES===
=== PUSH IMAGES ===
* WARNING: Only do this when LEO UIF software is shut down!
* WARNING: Only do this when LEO UIF software is shut down!
* Double click “Push [Your Name]” from list of icons in User Operations window.
* Double click “Push [Your Name]” from list of icons in User Operations window.
** Repeat this procedure WITHOUT LAUNCHING LEO UIF.
** Repeat this procedure WITHOUT LAUNCHING LEO UIF.
===PUMP CHAMBER===
=== PUMP CHAMBER ===
* Press V (if vacuum window not open).
* Press V (if vacuum window not open).
* Click Pump.
* Click Pump.
* If vacuum does not reach this level in 20 minutes, report to mailing list.
* If vacuum does not reach this level in 20 minutes, report to mailing list.
===VENT CHAMBER===
=== VENT CHAMBER ===
* Press V (if vacuum window not open).
* Press V (if vacuum window not open).
* Click Vent.
* Click Vent.
==TROUBLESHOOTING==
== TROUBLESHOOTING ==
===MOUSE TROUBLESHOOTING===
=== MOUSE TROUBLESHOOTING ===
* In case of erratic mouse movement:
* In case of erratic mouse movement:
** Lift mouse, shake it, blow into track ball
** Lift mouse, shake it, blow into track ball
** Click anywhere else within the image.
** Click anywhere else within the image.
===POWER / VACUUM TROUBLESHOOTING===
=== POWER / VACUUM TROUBLESHOOTING ===
* If Off light is illuminated:
* If Off light is illuminated:
** SEM likely lost power. Report loss of power to mailing list.
** SEM likely lost power. Report loss of power to mailing list.